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Western Blot analysis of K562 cells using Caspase-2 Polyclonal Antibody
Western Blot analysis of K562 cells using Caspase-2 Polyclonal Antibody
Western Blot analysis of K562 cells using Caspase-2 Polyclonal Antibody

CASP2 Antibody

CSB-PA001230
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCASP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CASP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID835
  • Target name
    CASP2
  • Target description
    caspase 2
  • Target synonyms
    CASP-2, ICH1, MRT80, NEDD-2, NEDD2, PPP1R57, caspase-2, caspase 2 apoptosis-related cysteine peptidase, neural precursor cell expressed developmentally down-regulated protein 2, protease ICH-1, protein phosphatase 1, regulatory subunit 57
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42575
  • Protein Name
    Caspase-2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Caspase-2 using anti-Caspase-2 antibody (A02384-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysate, Lane 2: human 22RV1 whole cell lysate, Lane 3: human SGC-7901 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-2 antigen affinity purified polyclonal antibody (Catalog # A02384-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-2 at approximately 51KD. The expected band size for Caspase-2 is at 51KD.
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