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Anti-Cathepsin D Antibody

Anti-Cathepsin D Antibody

A121574
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-Cathepsin D Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    3 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    Goat polyclonal antibody to Cathepsin D.
  • UNSPSC
    12352203

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Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Cathepsin D/CTSD Polyclonal Antibody (bs-1615R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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References
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Figure 1. IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9047). Cathepsin D was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Cathepsin D Antibody (PB9047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Immunohistochemical staining of human small intestine shows moderate to strong granular cytoplasmic positivity in glandular and lymphoid cells.
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