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Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells.

Anti-CBX3 Antibody

HPA004902
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCBX3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CBX3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11335
  • Target name
    CBX3
  • Target description
    chromobox 3
  • Target synonyms
    HECH, HP1-GAMMA, HP1Hs-gamma, HP1gamma, chromobox protein homolog 3, HP1 gamma homolog, chromobox homolog 3 (HP1 gamma homolog, Drosophila), heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, modifier 2 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13185
  • Protein Name
    Chromobox protein homolog 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of HP1 gamma using anti-HP1 gamma antibody (A01142). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysate, Lane 3: rat testis tissue lysate, Lane 4: rat PC-12 whole cell lysate, Lane 5: mouse brain tissue lysate, Lane 6: mouse brain tissue lysate, Lane 7: mouse testis tissue lysate, Lane 8: mouse HEPA1-6 whole cell lysate, Lane 9: mouse NIH/3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP1 gamma antigen affinity purified polyclonal antibody (Catalog # A01142) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP1 gamma at approximately 21KD. The expected band size for HP1 gamma is at 21KD.
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