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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-CCDC115 Antibody

HPA034598
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetVMA22
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CCDC115 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84317
  • Target name
    VMA22
  • Target description
    vacuolar ATPase assembly factor VMA22
  • Target synonyms
    CCDC115, CDG2O, ccp1, vacuolar ATPase assembly protein VMA22, coiled-coil domain containing 115, coiled-coil domain-containing protein 115
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96NT0
  • Protein Name
    Vacuolar ATPase assembly protein VMA22
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCDC115 using anti-CCDC115 antibody (A13807-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC115 antigen affinity purified polyclonal antibody (Catalog # A13807-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC115 at approximately 20 kDa. The expected band size for CCDC115 is at 20 kDa.
Product group Antibodies
Boster Bio
Anti-CCDC115 Antibody Picoband(r)A13807-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetVMA22
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Atlas Antibodies
PrEST Antigen CCDC115APREST79073
Protein IDQ96NT0
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Product group Antibodies
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CCDC115 AntibodyCSB-PA004636GA01HU
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetVMA22
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Product group Antibodies
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TargetVMA22
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