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CCDC115 Antibody

CSB-PA004636GA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVMA22
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCDC115 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84317
  • Target name
    VMA22
  • Target description
    vacuolar ATPase assembly factor VMA22
  • Target synonyms
    CCDC115, CDG2O, ccp1, vacuolar ATPase assembly protein VMA22, coiled-coil domain containing 115, coiled-coil domain-containing protein 115
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96NT0
  • Protein Name
    Vacuolar ATPase assembly protein VMA22
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCDC115 using anti-CCDC115 antibody (A13807-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC115 antigen affinity purified polyclonal antibody (Catalog # A13807-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC115 at approximately 20 kDa. The expected band size for CCDC115 is at 20 kDa.
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