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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.

Anti-CCDC36 Antibody

HPA045690
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetIHO1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CCDC36
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID339834
  • Target name
    IHO1
  • Target description
    interactor of HORMAD1 1
  • Target synonyms
    cancer/testis antigen 74; CCDC36; coiled-coil domain containing 36; coiled-coil domain-containing protein 36; CT74; interactor of HORMAD1 protein 1; LELA1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IYA8
  • Protein Name
    Interactor of HORMAD1 protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCDC36 using anti-CCDC36 antibody (A14775). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC36 antigen affinity purified polyclonal antibody (Catalog # A14775) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC36 at approximately 66KD. The expected band size for CCDC36 is at 66KD.
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