Figure 1. Western blot analysis of CCL18 using anti-CCL18 antibody (PB9023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human CCL18 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL18 antigen affinity purified polyclonal antibody (Catalog # PB9023) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL18 at approximately 19KD. The expected band size for CCL18 is at 19KD.
Figure 1. Western blot analysis of CCL18 using anti-CCL18 antibody (PB9023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human CCL18 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL18 antigen affinity purified polyclonal antibody (Catalog # PB9023) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL18 at approximately 19KD. The expected band size for CCL18 is at 19KD.
Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband(r)
PB9023
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCCL18
Overview
- SupplierBoster Bio
- Product NameAnti-CCL18/PARC Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for CCL18 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- Applications SupplierELI, WB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID6362
- Target nameCCL18
- Target descriptionC-C motif chemokine ligand 18
- Target synonymsalternative macrophage activation-associated CC chemokine 1; AMAC1; AMAC-1; CC chemokine ligand 18; CC chemokine PARC; C-C motif chemokine 18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); chemokine (C-C), dendritic; CKb7; DCCK1; DC-CK1; dendritic cell chemokine 1; macrophage inflammatory protein 4; MIP-4; PARC; pulmonary and activation-regulated chemokine; SCYA18; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated
- HostRabbit
- IsotypeIgG
- Protein IDP55774
- Protein NameC-C motif chemokine 18
- Scientific DescriptionBoster Bio Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband® catalog # PB9023. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Reactivity SupplierHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203