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Anti-CCL8 Antibody

130-10239
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCCL8
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CCL8 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6355
  • Target name
    CCL8
  • Target description
    C-C motif chemokine ligand 8
  • Target synonyms
    C-C motif chemokine 8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; monocyte chemoattractant protein 2; monocyte chemotactic protein 2; SCYA10; SCYA8; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); small-inducible cytokine A8
  • Host
    Rabbit
  • Protein IDP80075
  • Protein Name
    C-C motif chemokine 8
  • Scientific Description
    Rabbit anti-CCL8 Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MCP2/CCL8 using anti-MCP2/CCL8 antibody (A03237-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat small intestine tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP2/CCL8 antigen affinity purified polyclonal antibody (Catalog # A03237-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP2/CCL8 at approximately 22 kDa. The expected band size for MCP2/CCL8 is at 11 kDa.
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