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MCP2 antibody [35509.11]

GTX10391
GeneTex
ApplicationsWestern Blot, ELISA, Neutralisation/Blocking
Product group Antibodies
TargetCCL8
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Overview

  • Supplier
    GeneTex
  • Product Name
    MCP2 antibody [35509.11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-2 microg/ml. ELISA: 2.0 microg/ml. Neutralizing/Inhibition: 1-4 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    35509.11
  • Conjugate
    Unconjugated
  • Gene ID6355
  • Target name
    CCL8
  • Target description
    C-C motif chemokine ligand 8
  • Target synonyms
    C-C motif chemokine 8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; monocyte chemoattractant protein 2; monocyte chemotactic protein 2; SCYA10; SCYA8; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); small-inducible cytokine A8
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP80075
  • Protein Name
    C-C motif chemokine 8
  • Scientific Description
    MCP2 and MCP3 are members of the C-C, or beta chemokine class. Other chemokines in this group include C10, Eotaxin, HCC1, I309, JE, MCP1, MIP1 alpha, MIP1 beta and RANTES. These chemokines act primarily as chemoattractants and antibody does not cross-react with other activate monocytes, dendritic cells, T lymphocytes, natural killer cells, B lymphocytes, basophils and eosinophils. MCP2 and MCP3 were originally identified as monocyte chemotactic proteins produced by human MG63 osteosacrcoma cells. MCP2 and MCP3 are approximately 9 kD polypeptides of 76 amino acids. MCP2 shares 62% amino acid sequence homology with MCP1, MCP3 shares 71% homology with MCP1, and MCP2 shares 58% homology with MCP3.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • CaSR Antagonist (Calcilytic) NPS 2143 Hinders the Release of Neuroinflammatory IL-6, Soluble ICAM-1, RANTES, and MCP-2 from Abeta-Exposed Human Cortical Astrocytes. Chiarini A et al., 2020 Jun 2, Cells
    Read more

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Figure 1. Western blot analysis of MCP2/CCL8 using anti-MCP2/CCL8 antibody (A03237-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat small intestine tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP2/CCL8 antigen affinity purified polyclonal antibody (Catalog # A03237-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP2/CCL8 at approximately 22 kDa. The expected band size for MCP2/CCL8 is at 11 kDa.
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