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Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.

Anti-CCR9 Antibody

HPA066879
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCCR9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CCR9 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10803
  • Target name
    CCR9
  • Target description
    C-C motif chemokine receptor 9
  • Target synonyms
    CC-CKR-9, CDw199, GPR-9-6, GPR28, C-C chemokine receptor type 9, G protein-coupled receptor 28, chemokine (C-C motif) receptor 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51686
  • Protein Name
    C-C chemokine receptor type 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCR9 using anti-CCR9 antibody (M02348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR9 antigen affinity purified monoclonal antibody (Catalog # M02348) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR9 at approximately 42 kDa. The expected band size for CCR9 is at 42 kDa.
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