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Anti-CCT7 Antibody

Anti-CCT7 Antibody

A98251
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-CCT7 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to CCT7.
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CCT7 antibody (A08169-2). Overlay histogram showing HepG2 cells stained with A08169-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT7 Antibody (A08169-2,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 eta antibody [C1C3] (GTX101347) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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