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ProductsBack/Anti-CD105 [MJ7/18]

Anti-CD105 [MJ7/18]

Ab00623-2.0
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetEng
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD105 [MJ7/18]
  • Delivery Days Customer
    7
  • Application Supplier Note
    MJ7/18 can be used for FC, WB, IP and IHC (Baluk P, et al. 2003. Am. J. Pathol. 163:1801).
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    FC; WB; IP; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MJ7/18
  • Gene ID13805
  • Target name
    Eng
  • Target description
    endoglin
  • Target synonyms
    AI528660; AI662476; CD105; cell surface MJ7/18 antigen; En; Endo; endoglin; S-endoglin; transmembrane glycoprotein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ63961
  • Protein Name
    Endoglin
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    MJ7/18 was prepared by immunizing rats with inflamed mouse skin tissue. The antibody was selected immunohistologically for reactivity with endothelial cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD105 using anti-CD105 antibody (A02997-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD105 antigen affinity purified polyclonal antibody (Catalog # A02997-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD105 at approximately 95-105KD. The expected band size for CD105 is at 71KD.
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