Anti-CD127 [GR34]

Absolute Antibody

Anti-CD127 [GR34]

7

This antibody binds human IL-7R.

This IL-7R antagonist antibody is a recombinant engineered version of clone R34.34. The binding affinity of this antibody for human IL-7R was checked using ELISA. Antibody GR34 (mouse IgG1 version) was determined to inhibit pSTAT5 signalling with an IC50of 0.22microg/ml. The mouse IgG1 version had an affinity for the IL-7Ralpha EDC of 15.3nM (KD) and a Kd of 8.75X10-4. The mouse IgG1 version was capable of binding to IL-7Ralpha expressed on CHO cells with an EC50 of 0.27microg/ml, and blocked IL-7/IL-7Ralpha with an IC50of 2.29microg/ml (WO2010017468).

ELISA, Neutralisation/Blocking, Other Application

antagonist; ELISA; block

Research Use Only

Monoclonal

GR34

IL7R

interleukin 7 receptor

CD127; CD127 antigen; CDW127; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL7RA; IL-7R-alpha; ILRA; interleukin 7 receptor alpha chain; interleukin-7 receptor subunit alpha

Rabbit

IgG

Interleukin-7 receptor subunit alpha

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original parental clone was generated by immunizing BALB/c mice with Pre-Alp, a t(l;19) pre-B leukemic cell line.

-20°C,2°C to 8°C

12352203