Anti-CD134 [A10-F]

Absolute Antibody

Anti-CD134 [A10-F]

7

This antagonist antibody is a humanized version of the original murine parent antibody A10. The binding specificity of this antibody to OX40 was determined by ELISA and flow cytometric techniques. Naive CD4+ T-cell assay was used to test the ability to inhibit cytokine production, proliferation, and to induce apoptosis in human CD4+ T-cells. A10-F inhibited cytokine release (IL-2/IL-5/IL-13) and CD4+ T-cells proliferation to the same level as its murine parent. A10-F was also tested for its antagonistic activities like inhibiting the proliferation and cytokine release of Th1 primed T-cells, Th2 primed T-cells and Th17 cells in the presence of irradiated OX40L transfected L-cells or parental L-cells. Under Th1 conditions, A10-F inhibited the production of IL-2, IL-5, IL-13 cytokines and cell proliferation. Under Th2 conditions, A10-F showed an antagonistic effect on activated CD4+ T-cells by inhibiting their proliferation and cytokine release (IL-2, IL-5, and IL-13). With Th17 cells, A10-F increased apoptosis in a dose dependent manner. Cell proliferation and cytokine production in Th2 cells primed with TSLP-activated myeloid CD11c+ dendritic cells (DCs) was inhibited in the presence of A10-F. It also further caused significant increase in apoptosis and a lower expression of Survivin. A10-F was also tested for its antagonistic activities and the ability to inhibit the proliferation and cytokine production of Th1 primed T-cells in the presence of LPS-activated DCs. Here also A10-F inhibited the IL-2, IFN-gamma, and TNF-alpha cytokines production and cell proliferation (US20100136030).

Flow Cytometry, ELISA, Neutralisation/Blocking

ELIS; FC; Block

Research Use Only

Monoclonal

A10-F

TNFRSF4

TNF receptor superfamily member 4

ACT35; ACT35 antigen; ATC35 antigen; CD134; CD134 antigen; IMD16; lymphoid activation antigene ACT35; OX40; OX40 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; tumor necrosis factor receptor superfamily member 4; TXGP1L

Human

IgG1

Tumor necrosis factor receptor superfamily member 4

Human, Cynomolgus Monkey, Rhesus Monkey

The original antibody was generated by immunizing mice with a soluble OX40/Fcgamma1 as an immunogen, combined with Freund's adjuvant (1:1) and administered to two six-week-old A/J mice.

-20°C,2°C to 8°C

12352203