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Anti-CD134 [OX-40]

Ab00547-8.1
Absolute Antibody
ApplicationsFunctional Assay, Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetTnfrsf4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD134 [OX-40]
  • Delivery Days Customer
    7
  • Application Supplier Note
    OX-40 antibody results in Fc-dependent (i.e. not Fab or F(ab)2) stimulation of in vitro activated T cell proliferation, but is not mitogenic for resting cells. This antibody can also label CD4+ T blasts and can pull down CD134/CD134+ cells.
  • Applications
    Functional Assay, Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    FC; functional; FACS; IHC-fr; IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-40
  • Gene ID25572
  • Target name
    Tnfrsf4
  • Target description
    TNF receptor superfamily member 4
  • Target synonyms
    MRC OX40; Ox40; OX40 antigen; OX40L receptor; Tax-transcriptionally activated glycoprotein 1; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; tumor necrosis factor superfamily, member 4; Txgp1
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDP15725
  • Protein Name
    Tumor necrosis factor receptor superfamily member 4
  • Scientific Description
    This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Rat
  • Reactivity Supplier Note
    OX40 antibody was prepared by immunizing mice with phytohaemagglutinin-activated rat lymph node cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
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