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ProductsBack/Anti-CD138 [587CT7.3.6.5.2]

Anti-CD138 [587CT7.3.6.5.2]

AB02725-21.0-BT
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSDC1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD138 [587CT7.3.6.5.2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The screening of hybridomas for specific antibodies was done using ELISA. Further confirmation of the specificity for CD138 was done using western blotting. This antibody was also used for immunofluorescent staining and Flow cytometric analysis of CD138 exoression on HepG2, T47D and U266 cells (CN104059151).
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    587CT7.3.6.5.2
  • Gene ID6382
  • Target name
    SDC1
  • Target description
    syndecan 1
  • Target synonyms
    CD138, SDC, SYND1, syndecan, syndecan-1, CD138 antigen, heparan sulfate proteoglycan fibroblast growth factor receptor, syndecan proteoglycan 1
  • Host
    Mouse
  • Isotype
    IgM
  • Protein IDP18827
  • Protein Name
    Syndecan-1
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Syndecan-1/SDC1 using anti-Syndecan-1/SDC1 antibody (A00991-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syndecan-1/SDC1 antigen affinity purified polyclonal antibody (Catalog # A00991-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syndecan-1/SDC1 at approximately 60KD. The expected band size for Syndecan-1/SDC1 is at 32KD.
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