Anti-CD138 [OC-46F2]

Absolute Antibody

Anti-CD138 [OC-46F2]

9

This antibody is specific for the extracellular domain of human syndecan-1. Syndecan-1 is a cell membrane protein that, after its shedding by heparanase enzymes, is accumulated in the extracellular matrix of some tumours, e.g. myeloma and lung carcinoma,

The original antibody inhibited vascular maturation and tumour growth in an experimental human melanoma model. The therapeutic efficacy of this antibody was also demonstrated in an experimental ovarian carcinoma model. OC-46F2 scFv version was used as a capture antibody in a solid phase sandwich ELISA (PMID: 26460958). The scFv version of this antibody was also used in the immunofluorescent and immunohistochemical analysis of melanoma tissues, human ovarian carcinomas, human normal ovary cells and SKMEL28 cells (PMID: 26460958, 26460958). Syndecan-1 expression on a panel of cell lines was analysed by flow cytometry using recombinant OC-46F2. The scFv antibody can also recognize purified human recombinant extracellular portion of syndecan-1 in a western blot assay. Mice treated with OC-46F2 scFv showed no co-distribution of syndecan-1 with vascular endothelial growth factor receptor 2 (VEGFR2), which is generally seen in intratumour melanoma microenvironment. (PMID: 23352437). Melanoma cells lose their ability to form tubule-like structures in vitro after blocking syndecan-1 using OC-46F2. In a human melanoma xenograft model the combined therapy using OC-46F2 and L19-IL2, an immunocytokine specific for the tumor angiogenic-associated B-fibronectin isoform(B-FN), led to a complete inhibition of tumor growth until day 90 from tumor implantation in 71% of treated mice (PMID: 26460958). The small immuno protein version of this antibody 46F2SIP was used in combination with L19-IL2 for combination therapy in a human ovarian carcinoma model (PMID: 31443604).

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

OC-46F2

SDC1

syndecan 1

CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; SDC; SYND1; syndecan; syndecan proteoglycan 1; syndecan-1

Syndecan-1

Human

-20°C,2°C to 8°C

12352203