Binding of anti-CD152/APC to recombinant CD152-coated CML beads. Methods: Anti-CD152/APC was tested for binding to CD152-muIg-coated 4micro CML beads in FACS (Inquire). Five x 10^5 CD152-muIg coated beads per tube were spun down and incubated 45 min at 4°C with 80microl of anti-CD152/APC at a 1:50 dilution (10microg/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 1.92 log10 fluorescent units when compared to a Mouse IgG1/APC Isotype control (#ANC-278-060). Binding was blocked when beads were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD152 antibody (#ANC-359-020).
Binding of anti-CD152/APC to recombinant CD152-coated CML beads. Methods: Anti-CD152/APC was tested for binding to CD152-muIg-coated 4micro CML beads in FACS (Inquire). Five x 10^5 CD152-muIg coated beads per tube were spun down and incubated 45 min at 4°C with 80microl of anti-CD152/APC at a 1:50 dilution (10microg/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 1.92 log10 fluorescent units when compared to a Mouse IgG1/APC Isotype control (#ANC-278-060). Binding was blocked when beads were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD152 antibody (#ANC-359-020).
anti-CD152 [CTLA-4] (human), mAb (ANC152.2/8H5) (APC)
ANC-359-060
Product group Antibodies
Overview
- SupplierAncell Corporation
- Product Nameanti-CD152 [CTLA-4] (human), mAb (ANC152.2/8H5) (APC)
- Delivery Days Customer10
- CertificationResearch Use Only
- ClonalityMonoclonal
- Scientific DescriptionHuman CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. - Monoclonal Antibody. Antibody clone ANC152.2/8H5 reacts with an extracellular epitope of human CD152 (CTLA-4) expressed on activated T cells. It has been reported to cross-react with dog, pig, cow, and cat CD152. Isotype: Mouse IgG1kappa. Immunogen: Stimulated human PBLs and human CD152 Ig fusion protein. Applications: ELISA, FACS, FUNC (Blocking). Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation.
- Storage Instruction2°C to 8°C
- UNSPSC12352203
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