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ProductsBack/anti-CD152 [CTLA-4] (human), mAb (ANC152.2/8H5) (preservative free)

anti-CD152 [CTLA-4] (human), mAb (ANC152.2/8H5) (preservative free)

ANC-359-820
Ancell Corporation
ApplicationsFlow Cytometry, ELISA, Neutralisation/Blocking
Product group Antibodies
ReactivityBovine, Canine, Feline, Human, Porcine
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Overview

  • Supplier
    Ancell Corporation
  • Product Name
    anti-CD152 (human), mAb (ANC152.2/8H5) (preservative free)
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry, ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ANC152.2/8H5
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. - Monoclonal Antibody. Isotype: Mouse IgG1kappa. Clone: ANC152.2/8H5. Applications: ELISA, FACS, FUNC (Blocking). 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation.
  • Reactivity
    Bovine, Canine, Feline, Human, Porcine
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Binding of anti-CD152/APC to recombinant CD152-coated CML beads. Methods: Anti-CD152/APC was tested for binding to CD152-muIg-coated 4micro CML beads in FACS (Inquire). Five x 10^5 CD152-muIg coated beads per tube were spun down and incubated 45 min at 4°C with 80microl of anti-CD152/APC at a 1:50 dilution (10microg/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 1.92 log10 fluorescent units when compared to a Mouse IgG1/APC Isotype control (#ANC-278-060). Binding was blocked when beads were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD152 antibody (#ANC-359-020).
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