Anti-CD1d [1B1]

AB02718-2.0
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCd1d1
Overview
- SupplierAbsolute Antibody
- Product NameAnti-CD1d [1B1]
- Delivery Days Customer9
- Antibody SpecificityThis antibody is specific for CD1d. Its binding is blocked by antibody clone 3D11. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
- Application Supplier NoteIn an attempt to further the understanding of CD1 proteins, flow cytometry was preformed on mouse spleen cells using the rat version of this antibody. Further, mice infected with L. monocytogenes were treated with the rat version of this antibody. Finally, mice spleen cells were stained with the mouse version of this antibody (Szalay et al, 1999; pmid:10358132). To investigate the tissue distribution of murine CD1 in mice, immunohistochemistry was preformed on thymus, small intestine, and spleen tissue of mice using the rat version of this antibody. Further, flow cytometry was preformed on epithelial cells and IEL using the rat version of this antibody (Brassay et al, 1997; pmid:9233616). In order to measure mCD1-restricted T cell responses to glycolipids, the rat version of this antibody was added after which cytokine levels were assessed by ELISA (Burdin et al, 1998; pmid:9759842). To assess the role of CD1 in the pathogenesis of lupus the rat version of this antibody was used for immunofluorescent staining and flow cytometry on single cell suspensions of mouse spleen cells or mouse bone marrow. The mouse version of this antibody was further tested in vivo on mice with lupus. Their lifespan showed an increase (Zeng et al, 2000; pmid:10799851). To study the expression of CD1 on B cell subsets from normal and b2m2/2 mice, single-cell suspensions of lymphoid cells from C57BL/6 and BALB/c mice were used for immunofluorescence using the mouse version of this antibody (Amano et al, 1998; pmid:9712035). To eludicate the location of expression of CD1 flow cytometry was preformed on thymocytes from wild-type, b2m-deficient, and TAP-1-deficient C57BL/6 mice (Roark et al, 1998; pmid:9531266).
- ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1B1
- Gene ID12479
- Target nameCd1d1
- Target descriptionCD1d1 antigen
- Target synonymsAI747460; antigen-presenting glycoprotein CD1d1; Cd1; CD1.1; Cd1a; Cd1d; Ly-3; Ly-38; T-cell surface glycoprotein CD1d1
- HostMouse
- IsotypeIgG2a
- Protein IDP11609
- Protein NameAntigen-presenting glycoprotein CD1d1
- Scientific DescriptionThis chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format for improved compatibility with existing reagents assays and techniques.
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203