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Anti-CD2 [BAG 23D6]

Ab01524-10.0
Absolute Antibody
TargetCD2
Product group Antibodies
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD2 [BAG 23D6]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The clone BAG 23D6 is recommended for the identification and analysis of CD2.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BAG 23D6
  • Gene ID914
  • Target name
    CD2
  • Target description
    CD2 molecule
  • Target synonyms
    CD2 antigen (p50), sheep red blood cell receptor; erythrocyte receptor; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP06729
  • Protein Name
    T-cell surface antigen CD2
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was raised by immunizing mice against CD2.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCR2 using anti-CCR2 antibody (A00158-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR2 antigen affinity purified polyclonal antibody (Catalog # A00158-6) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR2 at approximately 42KD. The expected band size for CCR2 is at 42KD.
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