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Anti-CD2

Y300104
Applied Biological Materials
ApplicationsWestern Blot, ELISA
Product group Antibodies
100 ul
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Overview

  • Supplier
    Applied Biological Materials
  • Product Name
    Anti-CD2
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ml
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    CD2 is found as a surface antigen found on all peripheral blood T-cells and the majority of NK cells. It interacts with lymphocyte function-associated antigen (LFA-3/CD58) on antigen presenting cells to optimize immune recognition or CD48/BCM1 in rodents to mediate adhesion between T-cells and other cell types. CD2 also acts as a co-stimulatory molecule on T and NK cells and its cytoplasmic domain is implicated in the signaling function. As a specific marker for T and NK cells which is also expressed by the great majority of T cell lymphomas and leukaemias, CD2 can be used to distinguish these conditions from B cell neoplasms.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCR2 using anti-CCR2 antibody (A00158-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR2 antigen affinity purified polyclonal antibody (Catalog # A00158-6) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR2 at approximately 42KD. The expected band size for CCR2 is at 42KD.
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