Anti-CD227 [175C5]

Absolute Antibody

Anti-CD227 [175C5]

7

This antibody is specific for human CD227. The alpha subunit of CD227 has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

To characterize 13 different ovarian cancer xenografts, immunohistochemistry was performed different human ovarian cancer cell lines using the mouse version of this antibody. It only stained most tissues moderately (Molthoff et al, 1991; pmid:1985883). To determine whether the appearance of tumour-associated macin epitopes can be explained by altered post translational modification of mucin in tumours, flow cytometry was used on EBY-immortalized B-cells and Burkitts lymphomas using the mouse version of this antibody (Jerome et al, 1992; pmid:1382849). To study three antigenic systems for their use as tumour toxins, a western blot was performed on the human mammary carcinoma cell line MBMDA-23 1 and the colon carcinoma cell line SW 1222 using the mouse version of this antibody (Wick and Groner, 1997; pmid:9459206).

Flow Cytometry, Western Blot, ImmunoHistoChemistry

IHC; WB; FC

Research Use Only

Monoclonal

175C5

MUC1

mucin 1, cell surface associated

ADMCKD; ADMCKD1; ADTKD2; breast carcinoma-associated antigen DF3; CA 15-3; cancer antigen 15-3; carcinoma-associated mucin; CD227; EMA; episialin; H23 antigen; H23AG; KL-6; krebs von den Lungen-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; mucin 1, transmembrane; mucin-1; peanut-reactive urinary mucin; PEM; PEMT; polymorphic epithelial mucin; PUM; tumor associated epithelial mucin; tumor-associated epithelial membrane antigen

Rabbit

IgG

Mucin-1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The mouse version of this antibody was raised by immunizing a BALB/c mouse with milkfat globule membranes.

-20°C,2°C to 8°C

12352203