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Immunohistochemistry analysis in human small intestine and skeletal muscle tissues using HPA003326 antibody. Corresponding CD2AP RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human small intestine and skeletal muscle tissues using HPA003326 antibody. Corresponding CD2AP RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human small intestine and skeletal muscle tissues using HPA003326 antibody. Corresponding CD2AP RNA-seq data are presented for the same tissues.

Anti-CD2AP Antibody

HPA003326
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCD2AP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CD2AP
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23607
  • Target name
    CD2AP
  • Target description
    CD2 associated protein
  • Target synonyms
    CMS, CD2-associated protein, Cas ligand with multiple Src homology (SH) 3 domains, Cas ligand with multiple Src homology 3 (SH3) domains, adapter protein CMS, cas ligand with multiple SH3 domains
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5K6
  • Protein Name
    CD2-associated protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CD2AP using anti-CD2AP antibody (A01756-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human COLO320 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat testis tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD2AP antigen affinity purified polyclonal antibody (Catalog # A01756-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD2AP at approximately 85 kDa. The expected band size for CD2AP is at 70 kDa.
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