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Western Blot Positive WB detected in: Rat kidney tissue All lanes: CD2AP antibody at 11ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: CD2AP antibody at 11ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: CD2AP antibody at 11ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa

CD2AP Antibody

CSB-PA004914LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetCD2AP
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD2AP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23607
  • Target name
    CD2AP
  • Target description
    CD2 associated protein
  • Target synonyms
    CMS, CD2-associated protein, Cas ligand with multiple Src homology (SH) 3 domains, Cas ligand with multiple Src homology 3 (SH3) domains, adapter protein CMS, cas ligand with multiple SH3 domains
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5K6
  • Protein Name
    CD2-associated protein
  • Scientific Description
    Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD2AP using anti-CD2AP antibody (A01756-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human COLO320 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat testis tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD2AP antigen affinity purified polyclonal antibody (Catalog # A01756-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD2AP at approximately 85 kDa. The expected band size for CD2AP is at 70 kDa.
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