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Anti-CD3 epsilon [145-2C11]

Ab00105-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, Other Application
Product group Antibodies
TargetCd3e
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD3 epsilon [145-2C11]
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, Other Application
  • Applications Supplier
    FC; WB; IP; activation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    145-2c11
  • Gene ID12501
  • Target name
    Cd3e
  • Target description
    CD3 antigen, epsilon polypeptide
  • Target synonyms
    AI504783; CD3; CD3epsilon; T3e; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP22646
  • Protein Name
    T-cell surface glycoprotein CD3 epsilon chain
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD3 epsilon/Cd3e using anti-CD3 epsilon/Cd3e antibody (A02765-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3 epsilon/Cd3e antigen affinity purified polyclonal antibody (Catalog # A02765-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3 epsilon/Cd3e at approximately 23 kDa. The expected band size for CD3 epsilon/Cd3e is at 21 kDa.
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