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ProductsBack/FITC-Linked Anti-T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Polyclonal Antibody

FITC-Linked Anti-T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Polyclonal Antibody

CAU35439
Biomatik
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd3e
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Overview

  • Supplier
    Biomatik
  • Product Name
    FITC-Linked Anti-T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12501
  • Target name
    Cd3e
  • Target description
    CD3 antigen, epsilon polypeptide
  • Target synonyms
    AI504783; CD3; CD3epsilon; T3e; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain
  • Host
    Rabbit
  • Protein IDP22646
  • Protein Name
    T-cell surface glycoprotein CD3 epsilon chain
  • Scientific Description
    The FITC-Linked Anti-T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: Flow cytometry.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Jurkat (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD3 epsilon Polyclonal Antibody (bs-0765R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of CD3 epsilon/Cd3e using anti-CD3 epsilon/Cd3e antibody (A02765-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3 epsilon/Cd3e antigen affinity purified polyclonal antibody (Catalog # A02765-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3 epsilon/Cd3e at approximately 23 kDa. The expected band size for CD3 epsilon/Cd3e is at 21 kDa.
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