Anti-CD32 [MDE-8]

Absolute Antibody

Anti-CD32 [MDE-8]

9

This antibody recognizes and binds Fc-gamma receptor IIa (FcgammaRIIa) or CD32a on peripheral blood monocytes, polymorphonuclear cells and platelets.

The binding ability of MDE-8 to FcgammaRIIa was confirmed using flow cytometry. MDE-8 binds to FcgammaRIIa on cell lines, peripheral bloodmonocytes, PMN, platelets and blocked ligand binding via this receptor. It can also effectively blocked FcgammaRIIa ligand-binding via its F(ab)2 fragment. Overnight incubation of monocytes with F(ab)2 fragments of MDE-8 leads to a 60% decrease in cell surface expression of FcgammaRIIa. MDE-8 whole antibody induced a concomitant 30% decrease of FcgammaRI on THP-1cells and monocytes (PMID: 15982355). MDE-8 was reoported to induce Fc-mediated hypothermia and severe thrombocytopenia in FCGR2A mice (PMID: 26396093).

Flow Cytometry, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

MDE-8

FCGR2A

Fc fragment of IgG receptor IIa

CD32; CD32A; CDw32; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc gamma receptor RIIa3; FCG2; fc-gamma-RIIa; FcGR; FCGR2; FCGR2A1; fcRII-a; IGFR2; igG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a

Human

IgG1

Low affinity immunoglobulin gamma Fc region receptor II-a

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203