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ProductsBack/Anti-CD33 [SGN-33 (Lintuzumab)]

Anti-CD33 [SGN-33 (Lintuzumab)]

AB00730-10.6
Absolute Antibody
ApplicationsFunctional Assay
Product group Antibodies
TargetCD33
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD33 [SGN-33 (Lintuzumab)]
  • Delivery Days Customer
    9
  • Application Supplier Note
    In vitro, this antibody is capable of mediating ADCC against AML cells (Caron, 1998). Though shown to be safe in phase III trials, the use of this antibody in combination with induction chemotherapy did not result in a statistically significant improvement in response rate or survival in patients with refractory/relapsed AML (Feldman, 2005).
  • Applications
    Functional Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SGN-33 (Lintuzumab)
  • Gene ID945
  • Target name
    CD33
  • Target description
    CD33 molecule
  • Target synonyms
    CD33 antigen (gp67); CD33 molecule transcript; gp67; myeloid cell surface antigen CD33; p67; sialic acid-binding Ig-like lectin 3; SIGLEC3; SIGLEC-3
  • Host
    Human
  • Protein IDP20138
  • Protein Name
    Myeloid cell surface antigen CD33
  • Scientific Description
    NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD33 using anti-CD33 antibody (PB9932). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SKOV3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD33 antigen affinity purified polyclonal antibody (Catalog # PB9932) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD33 at approximately 45 kDa. The expected band size for CD33 is at 40 kDa.
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