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Anti-CD34 [2E10]

AB01129-23.0-BT
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetCD34
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD34 [2E10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody effectively binds to CD34-expressing KG-1a cells in FC analysis, and has been shown to react specifically with CHO-CD34 cells, but not CHO cells (Qian et al, 2008). This antibody has also been shown to bind CD34 in western blot analysis (Qian et al, 2008).
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2E10
  • Gene ID947
  • Target name
    CD34
  • Target description
    CD34 molecule
  • Target synonyms
    CD34 antigen; hematopoietic progenitor cell antigen CD34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28906
  • Protein Name
    Hematopoietic progenitor cell antigen CD34
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
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