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Immunohistochemistry analysis in human placenta and cerebral cortex tissues using HPA036723 antibody. Corresponding CD34 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human placenta and cerebral cortex tissues using HPA036723 antibody. Corresponding CD34 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human placenta and cerebral cortex tissues using HPA036723 antibody. Corresponding CD34 RNA-seq data are presented for the same tissues.

Anti-CD34 Antibody

HPA036723
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD34
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CD34
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID947
  • Target name
    CD34
  • Target description
    CD34 molecule
  • Target synonyms
    hematopoietic progenitor cell antigen CD34, CD34 antigen, CD34 sialomucin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28906
  • Protein Name
    Hematopoietic progenitor cell antigen CD34
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
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