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Anti-CD3D Rabbit Monoclonal Antibody

ORB548302
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD3D
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-CD3D Rabbit Monoclonal Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    FC, ICC, IF, IHC, IP, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    IDE-3
  • Conjugate
    Unconjugated
  • Gene ID915
  • Target name
    CD3D
  • Target description
    CD3 delta subunit of T-cell receptor complex
  • Target synonyms
    CD3-DELTA, CD3DELTA, IMD19, T3D, T-cell surface glycoprotein CD3 delta chain, CD3 antigen, delta subunit, CD3 delta, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), OKT3, delta chain, T-cell receptor T3 delta chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04234
  • Protein Name
    T-cell surface glycoprotein CD3 delta chain
  • Scientific Description
    Anti-CD3D Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD3D using anti-CD3D antibody (A04405-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3D antigen affinity purified polyclonal antibody (Catalog # A04405-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3D at approximately 19 kDa. The expected band size for CD3D is at 19 kDa.
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