Anti-CD3e [Visilizumab (HuM291)]

Absolute Antibody

Anti-CD3e [Visilizumab (HuM291)]

7

This antibody is derived from its a murine parent antibody called M291. HuM291 was significantly less mitogenic to T cells in vitro as compared to OKT3 and induced the release of much lower levels of the cytokines tumor necrosis factor-alpha, interferon-gamma, and interleukin-10. HuM291 still retained the ability to modulate the CD3 complex and inhibit the mixed lymphocyte reaction. This antibody competes with OKT3 for binding to human T cells, which was detected using flow cytometry (PMID: 10480417). In vitro studies using chimpanzee peripheral blood mononuclear cells (PBMCs), HuM291 was reported to be less mitogenic than OKT3 and effectively depleted peripheral T cells without eliciting clinical signs of cytokine release syndrome, and recovered T cells were functionally normal (PMID: 10480415). Phase 1 trial of HuM291 in patients receiving renal allografts suggested that it was well tolerated and rapidly depleted circulating T cells in a dose-dependent manner and was associated with only mild to moderate symptoms of cytokine release (PMID: 11152101). Treatment with visilizumab (HuM291) in severe steroid-refractory ulcerative colitis induced symptomatic response and clinical response (PMID: 19714757). The (99m)Tc labeled version of this antibody is also recommended for imaging of human T cells and study of human T-lymphocyte trafficking and lymphocytic infiltration of tissues and organs (PMID: 19759100). This antibody is also reported to have some activity in advanced glucocorticoid-refractory acute graft-versus-host disease (GVHD) (PMID: 11929757).

Flow Cytometry

FC; Therapeutic; immunesuppression

Research Use Only

Monoclonal

Visilizumab (HuM291)

CD3E

CD3 epsilon subunit of T-cell receptor complex

CD3epsilon, IMD18, T3E, TCRE, T-cell surface glycoprotein CD3 epsilon chain, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain

Human

IgG1

T-cell surface glycoprotein CD3 epsilon chain

Human, Primate

Human, chimpanzee

The original antibody was generated by immunizing BALB/c mice with activated human T cells as immunogens.

-20°C,2°C to 8°C

12352203