Anti-CD4 [OKT4A]

Absolute Antibody

Anti-CD4 [OKT4A]

7

This antibody was used for flow cytometry on human peripheral blood mononuclear cells (Bach et al, 1981; pmid:6790623). This antibody was used in a complement- (C) mediated lysis of T cells (Bach et al, 1981; pmid:6790623). A humanized version of this antibody was used for flow cytometry on Human PBM. This was to test the binding affinity of the newly humanized antibody (Pulito et al, 1996; pmid:8609403). Regions of adult human brain tissue were obtained from a 31-year-old man at autopsy was infected with HIV and then immunostained with this antibody (Jordan et al ,1990; pmid:1702842). The effect of this antibody on immunosuppression in transplant patients was tested. The experiment was preformed in cynomolgus monkeys (Mourad et al, 1998; pmid:9521196). Immunoperoxidase was preformed with this antibody on frozen sections of lymph nodes and sequentially obtained allograft biopsies. The tissues used were obtained from cynomolgus monkeys (Mourad et al, 1998; pmid:9521196). The infection of HIV-1 was determined after binding to this antibody (Zeira et al, 1991; pmid:2034680). MT-4, U-937, and PBL cells were immunostained with this antibody (Schols et al, 1988; pmid:2566170). This antibody was used for treatment of chronic plaque psoriasis (Gottlieb et al, 2000; pmid:11004613).

Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry

FC; IF; IHC; functional assay; treatment;

Research Use Only

Monoclonal

OKT4A

CD4

CD4 molecule

CD4 antigen (p55); CD4 receptor; CD4mut; IMD79; OKT4D; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Human

IgG1

T-cell surface glycoprotein CD4

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

This antibody was raised by immunizing mice with human thymocytes.

-20°C,2°C to 8°C

12352203