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Anti-CD40 [A54 scFv]

AB00743-10.6-BS
Absolute Antibody
ApplicationsELISA, Neutralisation/Blocking, Other Application
Product group Antibodies
TargetCD40
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD40 [A54 scFv]
  • Delivery Days Customer
    9
  • Application Supplier Note
    A54 is able to block the CD40-CD154 interaction by around 60-80% and can also induce CD40 sinalling in human B cells when at unusually high concentrations (The CD40 receptor - target, tool and technology, Ellmark, 2002). This scFv can also be used for ELISA studies (PMID: 12153507)
  • Applications
    ELISA, Neutralisation/Blocking, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A54 scFv
  • Gene ID958
  • Target name
    CD40
  • Target description
    CD40 molecule
  • Target synonyms
    B cell surface antigen CD40; B cell-associated molecule; Bp50; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDW40; p50; TNFRSF5; tumor necrosis factor receptor superfamily member 5
  • Host
    Human
  • Protein IDP25942
  • Protein Name
    Tumor necrosis factor receptor superfamily member 5
  • Scientific Description
    This reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10051). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40/TNFRSF5 antigen affinity purified polyclonal antibody (Catalog # PB10051) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40/TNFRSF5 at approximately 43 kDa. The expected band size for CD40/TNFRSF5 is at 30 kDa.
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