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anti-CD40 (human), mAb (BE-1)

ANC-189-020
Ancell Corporation
ApplicationsFunctional Assay, Flow Cytometry, ImmunoPrecipitation, ELISA
Product group Antibodies
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Overview

  • Supplier
    Ancell Corporation
  • Product Name
    anti-CD40 (human), mAb (BE-1)
  • Delivery Days Customer
    10
  • Applications
    Functional Assay, Flow Cytometry, ImmunoPrecipitation, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BE-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Human CD40 is a member of the TNF receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 ligand) is essential for isotype switching. - Monoclonal Antibody. Isotype: Mouse IgG1. Clone: BE-1. Applications: ELISA, FACS, FUNC, IP. 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate Human CD40 is a member of the TNF receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 ligand) is essential for isotype switching.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Binding of anti-CD40/APC to human Raji cells. Methods: Five x 10^5 human Raji cells per tube were incubated 45 min on ice with 80microl of anti-CD40/APC at a dilution factor of 1:50 (10microg/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.94 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control (#ANC-278-050). Binding was blocked when cells were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD40 antibody (#ANC-300-020).
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