ProductsBack/anti-CD40 (human), mAb (EA-5) (APC)

Binding of anti-CD40/APC to human Raji cells. Methods: Five x 10^5 human Raji cells per tube were incubated 45 min on ice with 80microl of anti-CD40/APC at a dilution factor of 1:50 (10microg/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.94 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control (#ANC-278-050). Binding was blocked when cells were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD40 antibody (#ANC-300-020).

anti-CD40 (human), mAb (EA-5) (APC)

ANC-300-060

Product group Antibodies

Overview

Supplier
Ancell Corporation
Product Name
anti-CD40 (human), mAb (EA-5) (APC)
Delivery Days Customer
10
Certification
Research Use Only
Clonality
Monoclonal
Scientific Description
CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkins and Hodgkins lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary. - Monoclonal Antibody. Antibody clone EA-5 recognizes the human CD40 molecule. Isotype: Mouse IgG1. Immunogen: Murine L cells transfectants expressing human CD40. Applications: ELISA, FACS. CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkins and Hodgkins lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.
Storage Instruction
2°C to 8°C
UNSPSC
12352203

anti-CD40 (human), mAb (EA-5) (APC)

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