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Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in leukocytes.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in leukocytes.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in leukocytes.

Anti-CD40LG-25ul

HPA045827
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CD40LG-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC
  • Category Supplier
    Antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Gene ID
    P29965
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29965
  • Protein Name
    CD40 ligand
  • Scientific Description
    Rabbit Polyclonal Anti-CD40LG Antibody against Human CD40 ligand. Validated for Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (A01114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody (Catalog # A01114-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40L at approximately 19KD. The expected band size for CD40L is at 29KD.
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