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Anti-CD40LG Antibody

Anti-CD40LG Antibody

A39558
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-CD40LG Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.0 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to CD40LG
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (A01114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody (Catalog # A01114-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40L at approximately 19KD. The expected band size for CD40L is at 29KD.
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