Anti-CD43 [84-3C1]

Absolute Antibody

Anti-CD43 [84-3C1]

7

This antibody can be used in the identification of malignant lymphomas. CD43 is expressed on 22-37% of B-cell lymphomas and, at the same time, it is not expressed on reactive B-cells. Consequently, B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma. If used with an anti-CD20 antibody, this clone may be utilized in the immunophenotyping of the lymphomas in formalin-fixed tissues, as Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 usually indicates lymphoma. Importantly, 84-3C1 antibody can be used for the detection of CD43 via various methods. For instance, it was successful in indetifying that antigen in a lysate of dendritic cells platellet via Western blot (Julien et al., 2007). Finally, another group used this antibody during flow cytometric analysis a sample of B-cells (Yoon et al., 2017).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry

WB; FC; IP; IF; IHC

Research Use Only

Monoclonal

84-3C1

SPN

sialophorin

CD43; galactoglycoprotein; GALGP; GPL115; leukocyte sialoglycoprotein; leukosialin; LSN; sialophorin (gpL115, leukosialin, CD43)

Rabbit

IgG

Leukosialin

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Human

This antibody was raised by immunizng mice with stimulated human leukocytes.

-20°C,2°C to 8°C

12352203