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Anti-SPN Antibody

144-06412
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSPN
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SPN Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6693
  • Target name
    SPN
  • Target description
    sialophorin
  • Target synonyms
    CD43, GALGP, GPL115, LEU-22, LSN, leukosialin, galactoglycoprotein, leukocyte sialoglycoprotein, sialophorin (gpL115, leukosialin, CD43)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16150
  • Protein Name
    Leukosialin
  • Scientific Description
    SPN Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD43 using anti-CD43 antibody (PB9095). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD43 antigen affinity purified polyclonal antibody (Catalog # PB9095) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD43 at approximately 115KD. The expected band size for CD43 is at 40KD.
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