Anti-CD44 [F8:33]

Absolute Antibody

Anti-CD44 [F8:33]

9

To test the ability of the mouse version of this antibody to bind to CD44vRA, flow cytometry was preformed on Namalwa cells expressing different isoforms of CD44 (Golan et al, 2007; pmid:17383158). Further, it was used in ELISA preformed on different isotypes of the CD44 protein to assess the ability of this antibody to bind specific types of CD44; CD44vRA (CD44 from rheumatoid arthritis patients) was bound most strongly (Golan et al, 2007; pmid:17383158). Furthermore, flowcytometry was preformed to analyse apoptosis of synovial fluid cells. The mouse version of this antibody was used to induced apoptosis (Golan et al, 2007; pmid:17383158). It was administred to arthritic mice to reduce joint inflammation and induced apoptosis above the spontaneous level in the spleen of the mice (Golan et al, 2007; pmid:17383158). Transwell migration assay was preformed to assess the specificity of this antibody. It prevented namalwa-CD44vRA cells from crossing but did not prevent namalwa cells transfected with other forms of CD44 from crossing (US2011207678). Finally, flow cytometry was preformed with this antibody on synovial fluid cells and PBLs. The antibodies bound to the synovial fluid cells but not the PBLs from the same patient (Golan et al, 2007; pmid:17383158).

Functional Assay, Flow Cytometry, ELISA

Research Use Only

Monoclonal

F8:33

CD44

CD44 molecule (IN blood group)

CDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, CD44 molecule (Indian blood group), GP90 lymphocyte homing/adhesion receptor, Hermes antigen, In(Lu) related-p80, Indian blood group antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, epican, extracellular matrix receptor III, hematopoietic cell E- and L-selectin ligand, heparan sulfate proteoglycan, homing cell adhesion molecule, homing function and Indian blood group system, hyaluronate receptor, phagocyte glycoprotein 1, phagocytic glycoprotein 1, soluble CD44

Mouse

IgG2a

CD44 antigen

Human

-20°C,2°C to 8°C

12352203