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anti-CD44 (human), mAb (BU52) (F(ab)2)

ANC-193-520
Ancell Corporation
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Ancell Corporation
  • Product Name
    anti-CD44 (human), mAb (BU52) (F(ab)2)
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BU52
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Human CD44 molecules appear to serve as lymphocyte adhesion molecules with numerous functions in extracellular matrix binding, cell migration, hemopoisis and lymphocyte homing. - Monoclonal Antibody. Isotype: Mouse IgG1kappa. Clone: BU52. Applications: FACS. 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl Human CD44 molecules appear to serve as lymphocyte adhesion molecules with numerous functions in extracellular matrix binding, cell migration, hemopoisis and lymphocyte homing.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Binding of anti-CD44 Fab + GAM/FITC to human Molt-4 cells. Method: Five x 10^5 cultured human Molt-4 cells were incubated 45 minutes on ice with 80 microl of anti-CD44 Fab at 5 microg/ml. Cells were washed twice and incubated with 2nd reagent goat anti-Mouse IgG/FITC (#ANC-232-011), after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG1 Fab (#ANC-278-580).
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