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anti-CD44 (human), mAb (BU52) (FITC)

ANC-193-040
Ancell Corporation
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Ancell Corporation
  • Product Name
    anti-CD44 (human), mAb (BU52) (FITC)
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BU52
  • Conjugate
    FITC
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Human CD44 molecules appear to serve as lymphocyte adhesion molecules with numerous functions in extracellular matrix binding, cell migration, hemopoisis and lymphocyte homing. - Monoclonal Antibody. Isotype: Mouse IgG1kappa. Clone: BU52. Applications: FACS. 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). Human CD44 molecules appear to serve as lymphocyte adhesion molecules with numerous functions in extracellular matrix binding, cell migration, hemopoisis and lymphocyte homing.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Binding of anti-CD44 Fab + GAM/FITC to human Molt-4 cells. Method: Five x 10^5 cultured human Molt-4 cells were incubated 45 minutes on ice with 80 microl of anti-CD44 Fab at 5 microg/ml. Cells were washed twice and incubated with 2nd reagent goat anti-Mouse IgG/FITC (#ANC-232-011), after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG1 Fab (#ANC-278-580).
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