More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CD47 Antibody (clone MIAP410)

CD47 Antibody (clone MIAP410)

LS-C770026
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetCD47
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD47 Antibody (clone MIAP410)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 2.0 microg per 10E6 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 2.0 µg per 10E6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MIAP410
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID961
  • Target name
    CD47
  • Target description
    CD47 molecule
  • Target synonyms
    antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; integrin-associated signal transducer; leukocyte surface antigen CD47; MER6; OA3; Rh-related antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Anti-CD47 Antibody
Product group Antibodies
Antibodies.com
Anti-CD47 AntibodyA101512
ApplicationsWestern Blot, ELISA
  • SizePrice
FluoSite(tm) Anti-CD47 Antibody, PE-Labeled
Product group Antibodies
BPS Bioscience
FluoSite(tm) Anti-CD47 Antibody, PE-Labeled79105
TargetCD47
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CD47 Antibody144-01838
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetCD47
  • SizePrice
Figure 1. Western blot analysis of CD47 using anti-CD47 antibody (A00360-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD47 antigen affinity purified polyclonal antibody (Catalog # A00360-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD47 at approximately 41KD. The expected band size for CD47 is at 35KD.
Product group Antibodies
Boster Bio
Anti-CD47 Antibody Picoband(r)A00360-1-CARRIER-FREE
ApplicationsWestern Blot
TargetCD47
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-CD47 [mIAP430]Ab00738-10.0
ApplicationsImmunoFluorescence, Neutralisation/Blocking
TargetCD47
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-CD47EB12501
ApplicationsWestern Blot, ELISA
TargetCD47
  • SizePrice
Product group Antibodies
Biomatik
Integrin Associated Protein (IAP) Polyclonal AntibodyCAU29954
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCD47
  • SizePrice
IHC image of CSB-PA004940LA01HU diluted at 1:300 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
CD47 AntibodyCSB-PA004940LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCD47
  • SizePrice
Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using AMAb91440 antibody. Corresponding CD47 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-CD47 AntibodyAMAB91440
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetCD47
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-CD47 Recombinant Monoclonal Antibody [BLR131H]A700-131
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCD47
  • SizePrice