Bio-Connect
Figure 1. Western blot analysis of CD48 using anti-CD48 antibody (A03281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD48 antigen affinity purified polyclonal antibody (Catalog # A03281-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD48 at approximately 39KD. The expected band size for CD48 is at 28KD.
Figure 1. Western blot analysis of CD48 using anti-CD48 antibody (A03281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD48 antigen affinity purified polyclonal antibody (Catalog # A03281-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD48 at approximately 39KD. The expected band size for CD48 is at 28KD.
Figure 1. Western blot analysis of CD48 using anti-CD48 antibody (A03281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD48 antigen affinity purified polyclonal antibody (Catalog # A03281-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD48 at approximately 39KD. The expected band size for CD48 is at 28KD.

Anti-CD48 Antibody Picoband(r)

A03281-1-CARRIER-FREE
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
TargetCD48
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CD48 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID962
  • Target name
    CD48
  • Target description
    CD48 molecule
  • Target synonyms
    BCM1; BCM1 surface antigen; BLAST; BLAST1; B-lymphocyte activation marker BLAST-1; CD48 antigen; CD48 antigen (B-cell membrane protein); hCD48; leukocyte antigen MEM-102; mCD48; MEM-102; signaling lymphocytic activation molecule 2; SLAM family member 2; SLAMF2; TCT.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09326
  • Protein Name
    CD48 antigen
  • Scientific Description
    Boster Bio Anti-CD48 Antibody Picoband® catalog # A03281-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
TargetCD48
  • SizePrice
Product group Antibodies
Assay Genie
TargetCD48
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCD48
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCD48
  • SizePrice
Product group Antibodies
Absolute Antibody
TargetCD48
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence
TargetCD48
  • SizePrice
Anti-CD48 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
MedChem Express
TargetCD48
  • SizePrice
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA004941ESR1HU at dilution of 1:100
Product group Antibodies
Cusabio
CD48 AntibodyCSB-PA004941ESR1HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCD48
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-CD48Y058621
ApplicationsWestern Blot, ELISA
  • SizePrice