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CD48 Antibody (clone HM48-1)

LS-C770051
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetCD48
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD48 Antibody (clone HM48-1)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.25 microg per 10E6 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.. Flo, IHC, IP Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.25 µg per 10E6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HM48-1
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID962
  • Target name
    CD48
  • Target description
    CD48 molecule
  • Target synonyms
    BCM1; BCM1 surface antigen; BLAST; BLAST1; B-lymphocyte activation marker BLAST-1; CD48 antigen; CD48 antigen (B-cell membrane protein); hCD48; leukocyte antigen MEM-102; mCD48; MEM-102; signaling lymphocytic activation molecule 2; SLAM family member 2; SLAMF2; TCT.1
  • Host
    Hamster
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD48 using anti-CD48 antibody (A03281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD48 antigen affinity purified polyclonal antibody (Catalog # A03281-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD48 at approximately 39KD. The expected band size for CD48 is at 28KD.
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