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Anti-CD54 (ICAM-1) Antibody

Anti-CD54 (ICAM-1) Antibody

A40196
Antibodies.com
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-CD54 (ICAM-1) Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    Mouse monoclonal antibody to CD54 (ICAM-1)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Hela cells; Lane 2: HUVEC cells; Lane 3: Raji cells; Probed with ICAM1 (1G2) Monoclonal Antibody (bsm-52434R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Figure 1. Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 95 kDa. The expected band size for ICAM1 is at 95 kDa.
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