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Anti-ICAM1 [053-051]

AB04135-10.0
Absolute Antibody
ApplicationsFlow Cytometry, Other Application
Product group Antibodies
TargetICAM1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-ICAM1 [053-051]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody could detect ICAM1 in different cell lines (Liver cancer cell line HepG2, pulmonary adenocarcinoma cell line PC14) by FACS analysis. The IgG1 format of the antibody exhibited ADCC activity on NCI-H441, NCI-H1373 and HepG2 cell lines (US9388249B2).
  • Applications
    Flow Cytometry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    053-051
  • Gene ID3383
  • Target name
    ICAM1
  • Target description
    intercellular adhesion molecule 1
  • Target synonyms
    BB2; CD54; cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; ICAM-1; intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor; P3.58
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP05362
  • Protein Name
    Intercellular adhesion molecule 1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 95 kDa. The expected band size for ICAM1 is at 95 kDa.
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