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Anti-CD6 [OX-124]

Ab00538-1.1
Absolute Antibody
ApplicationsFlow Cytometry, Neutralisation/Blocking
Product group Antibodies
TargetCD6
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD6 [OX-124]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody blocks CD6/CD166 interactions; crossreacts well and less well on soluble recombinant rat and mouse CD6 respectively (Santos et al., 2019; pmid: 31162836).
  • Applications
    Flow Cytometry, Neutralisation/Blocking
  • Applications Supplier
    blocking; FC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-124
  • Gene ID923
  • Target name
    CD6
  • Target description
    CD6 molecule
  • Target synonyms
    CD6 antigen; T12; T-cell differentiation antigen CD6; TP120
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP30203
  • Protein Name
    T-cell differentiation antigen CD6
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was raised by immunizing BALB/c mice with a fusion protein consisting of human CD6 and rat CD4 domains 3 and 4 (CD6d3CD4d3 + 4) (corrected, see: (Hassan et al., 2019; pmid: 30890626)).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of T12/CD6 using anti-T12/CD6 antibody (A03913-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-T12/CD6 antigen affinity purified polyclonal antibody (Catalog # A03913-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for T12/CD6 at approximately 135 kDa. The expected band size for T12/CD6 is at 72 kDa.
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